|
KMID : 0358920060330030401
|
|
Journal of the Korean Academy of Pedodontics
2006 Volume.33 No. 3 p.401 ~ p.410
|
|
|
Generation of antibodies against N-terminus fragment of AgI/II protein from Streptococcus mutans GS-5
|
|
Han Ji-Hye
Kim Jae-Gon
Baik Byeong-Ju
Yang Yeon-Mi
Park Jeong-Yeol
|
|
|
Abstract
|
|
|
|
Dental caries results from localized demineralization of tooth enamel by acids of bacterial origin produced from the fermentation of dietary sugars. A group of related oral bacteria, collectively known as mutans streptococci, are implicated as the primary etiological agents of human caries. Within this group, Streptococcus mutans has been known as a causative agent for dental caries. As well as acid production yielding the demineralization of tooth enamel, adherence and colonization of S. mutans to the teeth are also important for their virulence Cell-surface fibrillar proteins, which mediate adherence to the salivary pellicle are virulence components of mutans streptococci, and primary candidates for a human caries vaccine. Here we report that the AgI/II gene from S. mutans GS-5 were cloned by PCR amplification of the bacterial chromosomal DNA and the integrity of cloned genes were confirmed by nucleotide sequencing. Sequence analyses showed the sequence alignment of 280 nucleotides between the cloned AgI/II and the reported sequence of S. mutans GS-5 showed the perfect match The cloned genes which signal nucleotide was truncated, were transferred into bacterial expression vector and the recombinant proteins were purified as His-tag fusion proteins In order to generate polyclonal antibodies against the recombinant proteins, AgI/II mr, some $100{\mu}g$ of the proteins was injected into mice three times. It can be used for an effective vaccine production to prevent dental caries caused by pathogenic S. mutans.
|
|
|
KEYWORD
|
|
|
Streptococcus mutans, AgI/II, Vaccine
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|